BIOTECHNOLOGY WORKSHOP at Hunter College
(BIOL 410 / 610)
- OVERVIEW: Techniques-intensive 4-credit, 4-week workshop in biotechnology techniques followed by a 3-month internship with stipend as well as job placement opportunities in a biotechnology company, biomedical research laboratory, or biotech-related field.
- ELIGIBILITY: Biology students who are interested must be enrolled in or have taken a course in molecular biology.
- WORKSHOP CURRICULUM: Project-oriented 4-credit course where students learn state-of-the-art skills in DNA recombinant technology. Lecture material focuses on the scientific foundation of each protocol and the broad medical application of DNA recombinant technology to areas such as therapeutics, gene therapy, diagnostics and forensics.
COURSE DESIGN AND PROTOCOLS: PROJECT- ORIENTED APPROACH
Objective #1 – Characterization and isolation of a cloned gene product
- Plasmid DNA transformation
- Restriction analyses and gel electrophoresis
- Preparation of biotin labeled probes from gel-purified restricted fragments
- Southern Blotting
- Protein purification by column chromatography
- Western Blotting
Objective #2 – Gene expression
- RNA isolation and analysis by gel electrophoresis
- RT-PCR with specific primers for expression of genes with pathological implications
- Gel purification and cloning of PCR products
- INTERNSHIP: Students who successfully complete the Workshop with a grade of B or better are eligible to acquire additional skills and gain work experience through paid internships for a period of 3 months.
- JOB PLACEMENT: This program helps students develop their resumes and to obtain a position in a biotech company, research laboratory or a biotechnology-related field.
- SCHEDULE: January Intersession and the month of June
- To Enroll in Course Contact:
Dr. Patricia Rockwell
Room 821 Hunter North
rockwell@genectr.hunter.cuny.edu
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